What is the first step in blood collection?

The first step to the collection is to positively identify the patient by two forms of identification; ask the patient to state and spell his/her name and give you his/her birth date.

What is the process of collecting blood?

Venipuncture is the most common way to collect blood from adult patients. Collection takes place from a superficial vein in the upper limb, generally the median cubital vein; this vein is close to the skin and doesn’t have many large nerves positioned close by. This reduces pain and discomfort for the patient.

What are the stages of processing a blood sample?

There are four steps involved in obtaining a good quality specimen for testing: (1) preparation of the patient, (2) collection of the specimen, (3) processing the specimen, and (4) storing and/or transporting the specimen.

What is the order of blood collection tubes?

Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first. or incubation conditions.

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What is the order of draw?

The “Order of Draw” is designed to eliminate the possibility of cross contamination that may result in erroneous results. It is based on CLSI Procedures for Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard Sixth Edition, October 2007.

Which vein is the first choice for venipuncture?

The antecubital area of the arm is usually the first choice for routine venipuncture. This area contains the three vessels primarily used by the phlebotomist to obtain venous blood specimens: the median cubital, the cephalic and the basilic veins.

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Location Placement
Reason for Choice

Why is order of draw important?

The order of draw is recommended for both glass and plastic venous collections tubes when drawing multiple specimens for medical laboratory testing during a single venipuncture. The purpose is to avoid possible test result error due to additive carryover. All additive tubes should be filled to their stated volumes.

What are the 3 main veins to draw blood?


The most site for venipuncture is the antecubital fossa located in the anterior elbow at the fold. This area houses three veins: the cephalic, median cubital, and basilic veins (Figure 1).

Why is sodium citrate collected first?

Blue Top – Tube contains Sodium Citrate.

Must be drawn prior to collection of serum tubes to prevent contamination with clot activator and interference with coagulation cascade.

How do you remember the order of draw?

A mnemonic device is a phrase or saying that is easy to remember and relate back to in the future. It’s a great way to remember order of draw (OOD) because you can modify the sentence to be most helpful to you. In this example, the tubes that make the order of draw are identified by color.

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Why is blood culture drawn first?

Blood cultures should be drawn prior to initiation of antimicrobial therapy. Preparation of skin prior to blood culture collection is important to prevent contamination of sample. At least two (2) sets of blood cultures should be obtained (each set includes one (1) aerobic and one (1) anaerobic bottle).

What is the first thing the phlebotomist should do?

What is the first thing the phlebotomist should do in the event of an accidental needlestick? Decontaminate the site and fill out and incident report. Which of the following is an example of a work practice control that reduces the risk of exposure to bloodborne pathogens?