Frequent question: Which reagent is used for occult blood test?

The current worldwide standard is the Hemoccult slide test (Smith-Kline Diagnostics), a guaiac-based test, in which one side of a guaiac-impregnated paper is smeared with stool and tested by addition of a few drops of developer solution (stabilized peroxide reagent) to the opposite side of the paper.

How is occult blood measured?

The fecal immunochemical test (FIT) is the test of choice over the fecal occult blood test. This test can be done at home. For this test, you take a sample from your stool with a brush and dab it onto a special card. This test may be easier to do at home than the FOBT.

Which method is used for detection of occult blood in urine?

1) Urine strip method

Urine strips are used in hematuria screening to test for occult blood.

What is another name for occult blood test?

Guaiac fecal occult blood test uses a chemical substance called guaiac to check for blood in the stool. Immunochemical fecal occult blood test uses an antibody to check for blood in the stool. Also called FOBT.

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What test uses the reagent guaiac?

A guaiac fecal occult blood test (FOBT) checks for occult (hidden) blood in the stool. Small samples of stool are placed on a special card and returned to a doctor or laboratory for testing.

What is the principle of occult blood test?

Principle: Hemocult is a screening procedure for fecal blood and consists of a cardboard slide with guaiac-impregnated paper in a cardboard frame which permits sample application to one side with development and interpretation on the reverse side.

What is occult blood?

Occult blood means that you can’t see it with the naked eye. Blood in the stool means there is likely some kind of bleeding in the digestive tract. It may be caused by a variety of conditions, including: Polyps.

What is a benzidine test?

Medical Definition of benzidine test

: a sensitive test for the presence of blood (as in urine or feces) based on the production of a blue color upon contact with a solution of benzidine, hydrogen peroxide, and glacial acetic acid.

What is a fit test kit?

You use a home test kit, called a faecal immunochemical test (FIT), to collect a small sample of poo and send it to a lab. This is checked for tiny amounts of blood. Blood can be a sign of polyps or bowel cancer. Polyps are growths in the bowel. They are not cancer, but may turn into cancer over time.

How do you treat occult blood in stool?

Treatment of bleeding most often involves endoscopic ablation of the bleeding site with thermal energy, if the site is accessible. Angiographic embolization may be used to treat lesions that cannot be reached endoscopically. Diffuse vascular lesions, which are not uncommon, are difficult to treat.

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How accurate is FOBT test?

The 6-sample FOBT and the single digital FOBT had specificities of 93.9% and 97.5%, respectively, as defined by studying 1656 patients with no neoplasia. Sensitivities for detection of advanced neoplasia in 284 patients were 23.9% for the 6-sample FOBT and 4.9% for the digital FOBT.

Why is a Hemoccult test done?

The hemoccult test is a home test that’s used to detect the presence of occult blood in your stool. Occult blood is blood in your stool that you can’t see in the toilet or on the toilet paper after you have a bowel movement. The hemoccult test is predominantly used as a diagnostic tool for colorectal cancer.

How do you take the Hemoccult test?


  1. Gather your supplies. …
  2. Open the large front flap of the Hemoccult slide. …
  3. Sit on the toilet like you usually do to have a bowel movement (poop). …
  4. Take a sample of your stool with one end of an applicator stick. …
  5. Use the stick to collect a second sample from a different part of your stool.

What is Hemoccult Sensa developer?

§ Hemoccult SENSA Developer solution contains a stabilized mixture of less than 4.2% hydrogen peroxide, 84% denatured ethyl alcohol and enhancer in aqueous solution. NOTE: Any lot number of developer may be used with any lot number of slides as long as both are within expiration date and are “color-matched.”

How sensitive is occult test?

For the occult blood tests, 39 (14.1%) patients were positive for both tests, while 198 (71.5%) were negative by both tests.

Table 4.

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Sensitivity 50.00% (6.76–93.24) 75.00% (19.41–99.37)
Specificity 77.87% (72.24–82.83) 90.12% (85.76–93.50)

What type of specimen is needed for a guaiac test?

The stool guaiac test looks for hidden (occult) blood in a stool sample.

In brief:

  • You collect a stool sample from 3 different bowel movements.
  • For each bowel movement, you smear a small amount of the stool on a card provided in the kit.
  • You mail the card to a laboratory for testing.